Michigan Sea Grant
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Activity Seven
Beetle Collection and Release

Objectives
Students will observe and investigate natural enemies of indigenous wetland plants or on-site indigenous school plants and compare them to the observable enemies of purple loose strife. This activity may be done as a field observation, hands-on classroom activity or research activity. Students will then release Galerucella beetles (a purple loosestrife natural enemy) in a local loosestrife infested area.

State Science Curriculum Objectives
Constructing objectives: 13, 14, 15
Reflecting objectives: 11, 13, 15
Using objectives LEC: 6, 7, 15, 16, 18

Methods
Students will locate an infested wetland for on-site purple loosestrife and indigenous plant herbivory observation and/or teacher/student will bring in samples of loose strife and indigenous plants to classroom for observation. Students will either shake down the plants for possible natural enemies and/or record signs of herbivory in the classroom and compare the natural enemies of indigenous plants to local purple loose strife. Students will then predict possible impacts on plant populations based on observed evidence. After learning about the purple loosestrife project and Galerucella beetles, students will release these natural enemies in the wetland area. 

Terms
Herbivory, predators, indigenous and nonindigenous species.

Background
Ecosystems evolve a balance between their many species. Predation is a part of that natural balance. Indigenous plant and animal populations are kept in check by indigenous predators. Invading species threaten ecosystems because invaders frequently do not have predators. The invaders often have the ability to disrupt the existing ecological balance, dominate an area and have adverse unforeseen consequences. Purple loosestrife is an invading species with few if any local natural predators.The USDA has approved theGalerucella beetle, a natural predator from Europe for use in Purple loosestrife control. Section Two of the Cooperator's Handbook provides details on the biology, ecology and management of these two organisms, and includes additional references for teachers and students. See: About field collection of Galerucella.

Materials
White bedsheets, scissors or plant clippers, small sealable plastic bags, and large plastic bags, tweezers, magnifying glasses, insect identification books, microscopes (dissection, field or Brock optical scopes), and a wetland location with purple loosestrife.

Procedure

  1. Locate a site with a fairly large, medium to heavy infestation of purple loosestrife. (Much of this activity can still be done if a site with just a few loosestrife is the only available site.) Arrange a field trip to the site. Obtain permission to enter the site.
  2. While on site, students individually or in groups of two, map and record site observations such as surrounding human activity, plant and animal life, water amount and flow etc. Students draw map noting surrounding roads and locating observed plants and animals water etc. On map note, time of day, year and weather observations. This may impact findings.
  3. Divide students into groups of 3-4 for collection. To collect data in the field, students choose several indigenous plants on the site to compare to the purple loosestrife found there. Sit or stand quietly and observe the plants for visitors that might be feeding. Record any observed insects or birds feeding on the plants. Collect predator data by shaking the plants over a sheet and putting contents of sheet into individually marked small sealable bags for later identification. Using the same plants record the observable signs of herbivory, holes and chewing on leaves, and stems. Use the same number of each plant and randomly observe the same number of leaves and stems for herbivory on each plant. Cut a sample or two of each plant to bring back to the classroom for further observation. To do data collection solely in the classroom, samples must be cut in the field and brought into the classroom for herbivory observation. Each specimen should be carefully bagged in the field to preserve any resident feeding insects. Each plant should be cut off at ground level.
  4. In the class room observe, identify and record different kinds and numbers of organisms found on the plants, including fungus. Using identification books try to identify organisms associated with the plant. Covering the desks or tables with sheets of white paper facilitates observation. Observe and record signs of herbivory on selected leaves and stems. The book Eco Inquiry, published by Kendall Hunt, is recommended for graphic examples of herbivory, in addition to the materials found in Section Two of theHandbook. Chart and compare data. Is there any difference between predation on indigenous plants and on the purple loose strife? Student may also do print material or internet research on their chosen plants about possible predators and other limits to growth.
  5. Present group findings to class with predictions for future population growth of the chosen plants compared to the purple loose strife based on data collected.
  6. Read and discuss the use of Galerucella beetles as predators for purple loosestrife control.
  7. Keep a running biannual observation log (spring and fall) of the beetle release site and how the herbivory relationship between purple loosestrife and other indigenous plants changes over time. This activity will become richer over the years. The same herbivory observation can be done year after year in the same place. It should be interesting to trace the impact of the beetles on the purple loosestrife and observe if the beetles impact any other plants in the area.

Extensions
A practice trial of this activity could be done with any plants in your local school yard.

Students of different school districts could provide information to the Purple Loosestrife Project office, using input forms provided. See the Purple Pages Fall and Spring forms or contact the project office for details.

Evaluation
Evaluation could be based on observation of students careful methodology with data collection, written record of observations and communication to class of predictions.

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11/01/2007
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